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Mouse/Rat CCL2/JE/MCP-1 Quantikine ELISA Kit, R&D Systems

A 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor.

Manufacturer: r & d systems  MJE00

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Catalog No. MJE00

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Description & Specifications


Gene ID (Entrez) 20296 (Mouse); 24770 (Rat)
Quantity 1 kit
Sensitivity 2pg/mL
Sample Type Cell Culture Supernates, Serum
Sample Volume Cell Culture Supernates (25μL), Serum (25μL)
Species Mouse, Rat
Sufficient For Sufficient materials to run ELISAs on at least two 96-well plates

MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (25μL), Serum (25μL)
Sensitivity: 2pg/mL
Specificity: Natural and recombinant mouse and rat MCP-1
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.