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eBioscience Mouse PrimeFlow™ RNA Assay

In situ hybridization assay that combines the power of the ViewRNA™ branched DNA technology with the single cell resolution of flow cytometry. Branched DNA technology uses pairs of target-specific probes to achieve 8,000 to 16,000 fold signal amplification from a desired mRNA transcript.

$4,132.50 - $4,132.50

Specifications

For Use With (Application) Flow Cytometric Analysis
Storage Requirements Storage conditions vary, see components list for details
Species Mouse
View More Specs
Includes:  PrimeFlow™ RNA Tubes
PrimeFlow™ RNA Fixation Buffer 1A
PrimeFlow™ RNA Fixation Buffer 1B
PrimeFlow™ RNA Permeabilization Buffer (10X)
PrimeFlow™ RNA Fixation Buffer 2 (8X)
PrimeFlow™ RNA Wash Buffer
PrimeFlow™ RNA Target Probe Diluent
PrimeFlow™ RNA PreAmp Mix
PrimeFlow™ RNA Amp Mix
PrimeFlow™ RNA Label Probe Diluent
PrimeFlow™ RNA Storage Buffer
Rnase Inhibitor 2 (100X)
PrimeFlow™ RNA Label Probes
Rnase Inhibitor 1 (1000X)
Positive Control Probe Sets:
Type 1 Mouse ACTB Alexa Fluor 647
Type 4 Mouse ACTB Alexa Fluor 488
Type 6 Mouse ACTB Alexa Fluor 750

 Alert

Contains Sodium azide and <10% formaldehyde

 Disclaimers

For Research Use Only

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501125096

 
ebioscience
8818001210
100 tests Each for $4,132.50
Description & Specifications

Specifications

For Use With (Application) Flow Cytometric Analysis
Storage Requirements Storage conditions vary, see components list for details
Species Mouse

  • Simultaneous detection of up to three RNA targets
  • Combine with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations
  • This Mouse PrimeFlow™ RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for mouse beta Actin (ACTB, NM_007393)
  • Target Probe sets for genes of interest are sold separately
  • For the study of human samples, use the Human PrimeFlow™ RNA Assay (cat. 88-18009)
  • For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately