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eBioscience™ Mouse LIF, Recombinant Protein

Mouse Recombinant Protein

$143.55 - $375.55

Specifications

Structural Form E. coli derived Ser24-Phe203 accession number NM_008501
Purity >98%
Purification Method SDS-PAGE
Molecular Weight 20kDa
Concentration 0.1mg/mL
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501128870

 
ebioscience™
14-8521-80
25μg Each for $375.55

5018530

 
ebioscience™
14-8521-62
5μg Each for $143.55
Description & Specifications

Specifications

Structural Form E. coli derived Ser24-Phe203 accession number NM_008501
Purity >98%
Purification Method SDS-PAGE
Molecular Weight 20kDa
Concentration 0.1mg/mL
Source E. coli
Accession Number NM_008501
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay
Formulation Sterile liquid; phosphate-buffered saline with 0.05% Tween-20 and 1% BSA, 0.22μm filtered.
Biological Activity Recombinant mouse LIF has been tested for induction of mIL-6 in the M1 cell line. The ED50 in this assay is 0.75-0.4ng/mL, which corresponds to a specific activity of 1.3 x 106 - 2.5 x 106 Units/mg. When used at 10ng/mL, this recombinant protein has also been found to support mouse embryonic stem cells in an undifferentiated state at same concentration.
For Use With (Application) Cytokine Bioassays
Conjugate Unlabeled
Regulatory Status RUO
Cross Reactivity Mouse
Gene Symbol leukocyte inhibitory factor, Leukemia inhibitory factor
Name LIF
Recombinant Yes

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.