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Invitrogen™ eBioscience™ Mouse IL-9 ELISA Ready-SET-Go!™ Kit, 2nd Generation

Specifically engineered for accurate and precise measurement of IL-9 protein levels from samples including serum, and cell culture supernatants. IL-9 is a 14 kd cytokine originally named P40 and identified by its proliferative effects on T cell population

Manufacturer:  Invitrogen™ LS88809222

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Catalog No. 501125213



Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X Concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards.

Description

Description

IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta.
Specifications

Specifications

P15247
Biotin
16198
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Mouse
1 hr 45 min
Mouse
Interleukin-9, T-cell growth factor P40
32-4,000 pg/mL
ELISA
96-well plates
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22; -76; and -86)
2 x 96 tests
32 pg/mL
Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
Il9
HRP
Documents

For Research Use Only