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Mouse IL-6, Recombinant Protein, eBioscience™

Recombinant Protein

Manufacturer:  Life Technologies LS14806162

 View more versions of this product

Catalog No. 501128715



Description

Description

Interleukin 6 is a cytokine secreted by T cells and macrophages, has shown to exhibit activity in response to tissue damage and infection. They have also been found to be involved in various other activities like development of blood, cancer, embryonic development, and regulation of the immune system. IL6 is included in a family of other cytokines which include ciliary neurotrophic factor, cardiotrophin-1, leukemia inhibitory factor and interleukin 11. IL-6 can bring about differentiation through a receptor system which requires a specific receptor and a signaling subunit, gp130. Description: Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a ∽21-28kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction. Applications Reported: Recombinant mouse IL-6 is biologically active and can promote proliferation of mouse B cell lines in culture. It is also used as a cytokine standard in mouse IL-6 ELISA. Applications Tested: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/mL. This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg. Source: E. coli expressed amino acids Phe 25-Thre 211 of mature mouse IL-6 accession # MMINTL6. Bioactivity: The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5 pg/mL. This corresponds to a specific activity of greater than or equal to 2 x 10e8 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The protein is methionylated at the N-terminal. The DTT reduced and non-reduced protein migrate as 19kDa polypeptides on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

P08505
Cytokine Bioassays, ELISA
protein with 0.1M glycine and no preservative; pH 3.0
The protein is methionylated at the N-terminal. The DTT reduced and non-reduced protein migrate as 19kDa polypeptides on SDS-PAGE.
3.0
Affinity chromatography
E. coli
RUO
Recombinant
Mouse IL-6
Control, ELISA, Functional Assay
Mouse
Ms
Recombinant
0.1mg/mL
Liquid
16193
Mouse IL-6
SDS-PAGE
5μg
-80°C
E. coli expressed amino acids Phe 25-Thre 211 of mature mouse IL-6 accession # MMINTL6
Less than 0.01ng/μg cytokine as determined by the LAL assay.
Il6
The ED50 of this protein, as measured by B9 cell proliferation assay, is less than or equal to 5pg/mL. This corresponds to a specific activity of greater than or equal to 2 x 108 Units/mg.
Unlabeled
E. coli
Documents

For Research Use Only.