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Thermo Scientific™ Pierce™ Mouse IL-6 ELISA Kits


Measure levels of mouse interleukin-6 in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EM2IL62

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Catalog No. ENEM2IL62

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Description & Specifications

Specifications

Assay Range 51 to 2000pg/mL
Description Mouse IL-6 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 2 x 50mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 2 plate kit
Sensitivity <7pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with mouse GM-CSF, IFN?g, IL-1aa, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12, or human IL-6. This kit has been shown to have a high level of cross-reactivity with recombinant rat IL-6
Sufficient For 2 x 96 microplate wells
Total Assay Time 4 hr.
Target Natural and recombinant mouse interleukin-6 (IL-6)

Assay IL-6 (mouse interleukin-6) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-6 ELISA Kits.

These mouse IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-6 (IL-6)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 51 to 2000pg/mL

  • Sensitivity: < 7pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse GM-CSF, IFNγ, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12, or human IL-6. This kit has been shown to have a high level of cross-reactivity with recombinant rat IL-6

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates.