Measure levels of mouse interleukin-5 in mouse serum and culture supernatant samples using 96-well plates.
$651.05 - $2,436.00
Assay IL-5 (mouse interleukin-5) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-5 ELISA Kits.
These mouse IL-5 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-5 (IL-5) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-5 antibody pairs and IL-5 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Target: Natural and recombinant mouse interleukin-5 (IL-5)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 20 to 320pg/mL
Sensitivity: < 5pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum
Total Assay Time: 3.5 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNγ, GM-CSF, TNFα, rat IL-5, rabbit IL-5 or human IL-5
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).