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Invitrogen™ eBioscience™ Mouse IL-33 ELISA Ready-SET-Go!™ Kit

Specifically engineered for precise measurement of mouse IL-33 protein levels from samples, including serum, and cell culture supernatants.

Manufacturer:  Invitrogen™ LS88733388

 View more versions of this product

Catalog No. 501125201



Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA Coating Buffer
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
Specifications

Specifications

Q8BVZ5
Biotin
77125
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: NUNC Maxisorp flat-bottom (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
10 x 96 tests
25 pg/mL
Serum (100μL), Supernatants (100μL)
2-8°C
24 hr 30 min
Il33
HRP
25-3,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA
RUO
Serum, Supernatants
Mouse
1 hr 45 min
Mouse
Interleukin-33
Documents

For Research Use Only