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Thermo Scientific™ Pierce™ Mouse IL-2 ELISA Kits


Measure levels of mouse interleukin-2 in mouse serum and cell culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EMIL25

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Catalog No. ENEMIL25

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Description & Specifications

Specifications

Assay Range 34 to 850pg/mL
Description Mouse IL-2 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 200mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 5 plate kit
Sensitivity <3pg/mL
Sample Type Cell culture supernatant, serum
Sample Size 50µL
Specificity No significant cross-reaction with mouse IL-3, IL-4,IL-5, IL-6, IL-10, IFNg, GM-CSF, TNFa or human IL-2
Sufficient For 5 x 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant mouse interleukin-2 (IL-2)

Assay IL-2 (mouse interleukin-2) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-2 ELISA Kits.

These mouse IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-2 (IL-2)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 34 to 850pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-3, IL-4,IL-5, IL-6, IL-10, IFNγ, GM-CSF, TNFα or human IL-2

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).