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Mouse IL-12 (total) ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of total mouse interleukin-12 (IL-12, p70 plus p40)

Manufacturer: pierce biotechnology inc  EMIL12TOT5

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Catalog No. EMIL12TOT5

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Description & Specifications


Sensitivity < 5pg/mL
Sufficient For 5 x 96 microplate wells
Measuring Range 12.3 to 1000pg/mL
Quantity 5 plate kit
Target Biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)
Test Time 3 hours
Specificity No significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF, or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 (p40) do not cross-react or interfere with this assay.
Precision Intra-assay CV < 10%; inter-assay CV < 10%
Sample Volume 50μL
Includes Anti-Mouse Total IL-12 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-12 Standard, 10 vials
Standard Diluent, contains 0.1% NaN3, 5 × 20mL
Biotinylated Antibody Reagent, contains 0.1% NaN3, 5 × 12mL
30X Wash Buffer, 5 × 50mL
Streptavidin-HRP Concentrate, 5 × 75μL
Streptavidin-HRP Dilution Buffer, 5 × 14mL
TMB Substrate, 5 × 13mL
Stop Solution, contains 0.16M sulfuric acid, 5 × 13mL
Adhesive plate covers, 30 each
Sample Type Cell culture supernatant, serum