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Mouse IL-12 (total) ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of total mouse interleukin-12 (IL-12, p70 plus p40)

Manufacturer: pierce biotechnology inc  EMIL12TOT5

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Catalog No. EMIL12TOT5


Description & Specifications


Includes Anti-Mouse Total IL-12 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-12 Standard, 10 vials
Standard Diluent, contains 0.1% NaN3, 5 × 20mL
Biotinylated Antibody Reagent, contains 0.1% NaN3, 5 × 12mL
30X Wash Buffer, 5 × 50mL
Streptavidin-HRP Concentrate, 5 × 75μL
Streptavidin-HRP Dilution Buffer, 5 × 14mL
TMB Substrate, 5 × 13mL
Stop Solution, contains 0.16M sulfuric acid, 5 × 13mL
Adhesive plate covers, 30 each
Precision Intra-assay CV < 10%; inter-assay CV < 10%
Quantity 5 plate kit
Sensitivity < 5pg/mL
Sample Type Cell culture supernatant, serum
Sample Volume 50μL
Specificity No significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF, or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 (p40) do not cross-react or interfere with this assay.
Sufficient For 5 x 96 microplate wells
Test Time 3 hours
Target Biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)
Measuring Range 12.3 to 1000pg/mL

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 47 to 3000pg/mL
  • Sensitivity: < 12pg/mL
  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12