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Thermo Scientific™ Pierce™ Mouse IL-12 (total) ELISA Kits

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ Pierce™ PIEMIL12TOT

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Catalog No. ENEMIL12TOT


 

Specifications

Description

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 47 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

<12pg/mL
No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
30X Wash Buffer, 50mL
Absorbance at 450nm minus absorbance at 550nm
Complete ELISA kit with coated 96-well plate
50µL
96 microplate wells
Dependent upon sample type (generally, intra- and inter-assay CV <10%)
1 plate kit
3 hr.
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
47 to 3000pg/mL
Mouse IL-12 Total (p70, p40) ELISA Kit
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate