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Thermo Scientific™ Pierce™ Mouse IL-12 (total) ELISA Kits


Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  PIEMIL12TOT

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Catalog No. ENEMIL12TOT

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Description & Specifications

Specifications

Sensitivity <12pg/mL
Precision Dependent upon sample type (generally, intra- and inter-assay CV <10%)
Specificity No significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
Quantity 1 plate kit
Target Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Total Assay Time 3 hr.
Kit Contents 30X Wash Buffer, 50mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Measurement Absorbance at 450nm minus absorbance at 550nm
Assay Range 47 to 3000pg/mL
Formulation Complete ELISA kit with coated 96-well plate
Description Mouse IL-12 Total (p70, p40) ELISA Kit
Sample Size 50µL
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Sufficient For 96 microplate wells

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 47 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.