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eBioscience™ Mouse IL-12, Recombinant Protein

Mouse Recombinant Protein

$303.05 - $839.55

Specifications

Structural Form Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, accession # NM_008352 was co-expressed with mouse p35, amino acids met 1-ala 215, accession # NM_008351
Purity >98%
Purification Method SDS-PAGE
Molecular Weight The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65kDa protein. The DTT reduced protein migrates as 43kDa and 24kDa polypeptides.
Source Insect cells infected with baculovirus
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 Disclaimers

For Research Use Only.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

501129086

 
ebioscience™
14-8121-62
5μg Each for $303.05

508974

 
ebioscience™
14-8121-80
25μg Each for $839.55
Description & Specifications

Specifications

Structural Form Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, accession # NM_008352 was co-expressed with mouse p35, amino acids met 1-ala 215, accession # NM_008351
Purity >98%
Purification Method SDS-PAGE
Molecular Weight The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65kDa protein. The DTT reduced protein migrates as 43kDa and 24kDa polypeptides.
Source Insect cells infected with baculovirus
Accession Number NM_008352 co-expressed with NM_008351
Endotoxin Concentration Less than 0.01ng/μg cytokine, as determined by the LAL assay.
Formulation Sterile liquid; 10mM NaH2PO4, pH 6.0, 300mM NaCl, with 1.0% BSA. 0.22μm filtered.
Biological Activity The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 106 Units/mg.
For Use With (Application) Cytokine Bioassays , ELISA Recombinant Standard
Conjugate Unlabeled
Regulatory Status RUO
Cross Reactivity Mouse
Gene Symbol Interleukin-12, IL12, p70
Name IL-12
Recombinant Yes

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience’s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.