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Thermo Scientific™ Pierce™ Mouse IL-12 (p70) ELISA Kits


Measure levels of active mouse interleukin-12, p70 in mouse serum and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EMIL122

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Catalog No. ENEMIL122

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Description & Specifications

Specifications

Assay Range 12.3 to 1000pg/mL
Description Mouse IL-12 (p70) ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96 well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 2 x 50mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 2 plate kit
Sensitivity <5pg/mL
Sample Type Cell culture supernatant, serum
Sample Size 50µL
Specificity No significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 < (p40) do not cross-react or interfere with this assay
Sufficient For 2 x 96 microplate wells
Total Assay Time 3 hr.
Target Biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)

Assay IL-12 p70 (active mouse interleukin-12, p70) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-12 (p70) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96 well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 12.3 to 1000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 < (p40) do not cross-react or interfere with this assay

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.