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Mouse IL-12 (p40) ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of mouse interleukin-12 p40 (IL-12 p70)

$2,436.00 - $2,436.00

Includes: Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

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EMIL12P405 Pierce Biotechnology Inc
Each for $2,436.00


These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically inactive interleukin-12 (IL-12 p40) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 12.5 to 800pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay