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Mouse IL-12 (p40) ELISA Kits, Thermo Scientific™

Microplate kits for sandwich ELISA of mouse interleukin-12 p40 (IL-12 p70)

Manufacturer: pierce biotechnology inc  EMIL12P405

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Catalog No. EMIL12P405

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Description & Specifications


Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50μL
Includes Anti-Mouse IL-12 p40 Pre-coated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-12 p40 Standard, 10 vials
Sample Diluent, contains 0.1% NaN3, 5 × 12mL
Biotinylated Antibody Reagent, contains 0.1% NaN3, 5 × 12mL
30X Wash Buffer, 5 × 50mL
Streptavidin-HRP Concentrate, 5 × 75μL
Streptavidin-HRP Dilution Buffer, 5 × 14mL
TMB Substrate, 5 × 13mL
Stop Solution, contains 0.16M sulfuric acid, 5 × 13mL
Adhesive plate covers, 30 each
Specificity No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay.
Sensitivity < 3pg/mL
Test Time 3 hours
Sufficient For 5 x 96 microplate wells
Quantity 5 plate kit
Measuring Range 12.5 to 800pg/mL
Precision Intra-assay CV < 10%; inter-assay CV < 10%
Target Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)