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Mouse IL-12 (p40) ELISA Kits, Thermo Scientific™


Microplate kits for sandwich ELISA of mouse interleukin-12 p40 (IL-12 p70)

Manufacturer: pierce biotechnology inc  EMIL12P405

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Catalog No. EMIL12P405

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Description & Specifications

Specifications

Includes Anti-Mouse IL-12 p40 Pre-coated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-12 p40 Standard, 10 vials
Sample Diluent, contains 0.1% NaN3, 5 × 12mL
Biotinylated Antibody Reagent, contains 0.1% NaN3, 5 × 12mL
30X Wash Buffer, 5 × 50mL
Streptavidin-HRP Concentrate, 5 × 75μL
Streptavidin-HRP Dilution Buffer, 5 × 14mL
TMB Substrate, 5 × 13mL
Stop Solution, contains 0.16M sulfuric acid, 5 × 13mL
Adhesive plate covers, 30 each
Precision Intra-assay CV < 10%; inter-assay CV < 10%
Quantity 5 plate kit
Sensitivity < 3pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50μL
Specificity No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay.
Sufficient For 5 x 96 microplate wells
Test Time 3 hours
Target Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)
Measuring Range 12.5 to 800pg/mL

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically inactive interleukin-12 (IL-12 p40) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 12.5 to 800pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay