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Thermo Scientific™ Pierce™ Mouse IL-12 (p40) ELISA Kits

Measure levels of inactive mouse interleukin-12, p40 in mouse serum, plasma and culture supernatant samples using 96-well plates.

Manufacturer:  Thermo Scientific™ Pierce™ EMIL12P402

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Catalog No. EEMIL12P402


 

Specifications

Description

Assay IL-12 p40 (inactive mouse interleukin-12, p40) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (p40) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically inactive interleukin-12 (IL-12 p40) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 12.5 to 800pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

Intra-assay CV <10%; inter-assay CV <10%
2 x 96 microplate wells
3 hr.
Natural and recombinant mouse interleukin-12 p40 (IL-12 p40)
50µL
Absorbance at 450nm minus absorbance at 550nm
No significant cross-reaction with mouse IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa or human IL-12 p40. Mouse IL-12 p70 exhibits 1% cross-reactivity in this assay
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
<3pg/mL
Mouse IL-12 (p40) ELISA Kit
30X Wash Buffer, 2 x 50mL
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
12.5 to 800pg/mL
2 plate kit
Complete ELISA kit with coated 96-well plates