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Thermo Scientific™ Pierce™ Mouse IL-10 ELISA Kits


Measure levels of mouse interleukin-10 in mouse serum and cell culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EM2IL10

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Catalog No. ENEM2IL10

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Description & Specifications

Specifications

Assay Range 37 to 3000pg/mL
Description Mouse IL-10 ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity <12pg/mL
Sample Type Cell culture supernatant, serum
Sample Size 50µL
Specificity No significant cross-reaction with mouse IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, IFNg, TNFa or human IL-10
Sufficient For 96 microplate wells
Total Assay Time 5 hr.
Target Natural and recombinant mouse interleukin-10 (IL-10)

Assay IL-10 (mouse interleukin-10) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-10 ELISA Kits.

These mouse IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 37 to 3000pg/mL

  • Sensitivity: < 12pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, IFNγ, TNFα or human IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.