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Thermo Scientific™ Pierce™ Mouse IL-1 beta ELISA Kits

Measure levels of mouse interleukin-1 beta (IL-1b) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

$651.05 - $1,145.50

Specifications

Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement Absorbance at 450nm minus absorbance at 550nm
Precision Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Target Natural and recombinant mouse interleukin-1 beta (IL-1ß)
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Products ${productFamilyLength}
Catalog Number Mfr. No. Sufficient For Price Quantity  
Catalog Number Mfr. No. Sufficient For Price Quantity  
ENEM2IL1B Thermo Scientific™ Pierce™
EM2IL1B
96 microplate wells Each for $651.05
ENEM2IL1B2 Thermo Scientific™ Pierce™
EM2IL1B2
2 x 96 microplate wells Each for $1,145.50
Specifications

Description

Assay IL-1β (mouse interleukin-1 beta) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 beta ELISA Kits.

These mouse IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant mouse interleukin-1 beta (IL-1β)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 31.2 to 2000pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-1α, IL-10, IL-2, IL-4, IL-6, TNFα, GM-CSF; human IL-1β, IL-8, IL-1α; or bovine IL-1β. This ELISA does cross-react with rat IL-1β (12%)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Natural and recombinant mouse interleukin-1 beta (IL-1ß)
Absorbance at 450nm minus absorbance at 550nm
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)