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Mouse IgG2a kappa, Super Bright 436, eBioscience™ DFS Item

Mouse Isotype Control

$172.00 - $385.00

Specifications

Antigen Mouse IgG2a kappa
Clone eBM2a
Target Species Mouse
Conjugate Super Bright 436
Isotype IgG2a
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
62472480 View Documents Life Technologies
LS62472480
25μg Each for $172.00
 
62472482 View Documents Life Technologies
LS62472482
100μg Each for $385.00
 
Description

Description

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis. Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. This should be used at the same concentration as experimental antibody. Super Bright 436 can be excited with the violet laser line (405nm) and emits at 436nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405nm; Emission: 436nm; Laser: Violet Laser

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody and quote;s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

Mouse IgG2a kappa
Mouse
IgG2a
Liquid
Isotype Control
4°C, store in dark, DO NOT FREEZE!
eBM2a
Super Bright 436
Control, Flow Cytometry
Control
PBS with BSA and 0.09% sodium azide; pH 7.2
RUO