Please login to your online account to display your discounted pricing

Mouse IgG1 Control


Mouse IgG1 Control from Thermo Scientific™ Pierce™ is validated for Ctrl, FACS and IHC (F).

Manufacturer: pierce biotechnology inc  MA518168

 View more versions of this product

Catalog No. PIMA518168

  • / Each
 

Description & Specifications

Specifications

Antigen Mouse IgG1
Applications Control
Applications Flow Cytometry
Clone MOPC-21
Conjugate Alexa Fluor 647
Format Purified
Formulation 100μg purified isotype control antibody (1mg/mL) in PBS and 15mM sodium azide; pH 7.4
Host Species Mouse
Isotype IgG1
Purification Method Size-exclusion chromatography
Quantity 100μg
Species Reactivity Not Applicable
Storage Requirements DO NOT FREEZE!

Mouse IgG1 Control from Thermo Scientific™ Pierce™ is validated for Ctrl, FACS and IHC (F).

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG1
Clone: MOPC-21
Formulation: 100μg purified isotype control antibody (1mg/mL) in PBS and 15mM sodium azide; pH 7.4
This product can be used as a negative control to measure the non-specific binding of Mouse IgG1 in human and rat tissues; it has been tested on a variety of resting, activated, live and fixed rat and human tissues.