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Thermo Scientific™ Pierce™ Mouse IFN gamma ELISA Kits


Measure levels of mouse interferon gamma (IFNg) in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  PIEM10012

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Catalog No. ENEM10012

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Description & Specifications

Specifications

Assay Range 37 to 3000pg/mL
Description Mouse IFN gamma ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plates
Kit Contents 30X Wash Buffer, 2 x 50mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 2 plate kit
Sensitivity <10pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with with mouse IL-1a, IL-1ß, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFa or human IFNa or IFNg
Sufficient For 2 x 96 microplate wells
Total Assay Time 4 hr.
Target Natural and recombinant mouse interferon gamma (IFNg)

Assay IFN-γ (mouse interferon gamma) levels in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Mouse IFN gamma ELISA Kits.

These mouse IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 37 to 3000pg/mL

  • Sensitivity: < 10pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).