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Thermo Scientific™ Pierce™ Mouse GM-CSF ELISA Kits


Measure levels of granulocyte macrophage colony-stimulating factor in mouse serum and culture supernatants using 96-well plates.

Manufacturer: thermo scientific™ pierce™  EMGMCSF

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Catalog No. ENEMGMCSF

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Description & Specifications

Specifications

Assay Range 0 to 250pg/mL
Description Mouse GM-CSF ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Reproducibility Intra-assay CV <= 10%; inter-assay CV <= 10%
Sensitivity <5pg/mL
Sample Type Cell culture supernatant, serum
Sample Size 50µL
Specificity No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, IFNg, TNFa, rabbit GM-CSF, rat GM-CSF or human GM-CSF
Sufficient For 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF)

Assay GM-CSF (granulocyte macrophage colony-stimulating factor) levels in mouse serum and culture supernatants with our Thermo Scientific™ Mouse GM-CSF ELISA Kits.

These mouse GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 0 to 250pg/mL

  • Sensitivity: < 5pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, serum

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, IFNγ, TNFα, rabbit GM-CSF, rat GM-CSF or human GM-CSF

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.