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Human IL-4 Animal Free, Recombinant Protein, eBioscience™
Manufacturer: Life Technologies LS68878063
DescriptionThe protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Description: This SAFE™ (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin. Human IL-4 is also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23. Applications Reported: SAFE™ Human IL-4 Recombinant Animal Free Protein is biologically active. Applications Tested: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.2ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg. Source: Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. accession number NP_000580. Bioactivity: The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 10e6 Units/mg. Endotoxin: Less than 0.005ng/ug (0.05 EU/ug) as measured by turbidity assay. Purity: Greater than or equal to 95% as determined by SDS-PAGE. Molecular Weight: 17.2kDa (apparent 23 and 24 kDa). Storage: The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile low salt buffer to a concentration no less than 100 ug/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots.
|Human IL-4 Animal Free|
|4°C or -20°C if preferred|
|Plant expressed 130 amino acids (His25-Ser153) with 16 amino acid Histidine tag. Accession number NP_000580.|
|Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay|
|The ED50 of this protein, as measured by TF-1 cell proliferation assay, is less than or equal to 0.7ng/mL. This corresponds to a specific activity of greater than or equal to 5 x 106 Units/mg.|
|PBS with no preservative; pH 7.2|
|17.2kDa (apparent 23 and 24kDa)|
|Human IL-4 Animal Free|
|Control, Functional Assay|