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Invitrogen™ Eotaxin Mouse ELISA Kit
For the quantitative detection of mouse Eotaxin.
Supplier: Invitrogen™ BMS6008TEN
Includes: Aluminum pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse Eotaxin
Biotin-Conjugate anti-mouse Eotaxin polyclonal antibody
Streptavidin-HRP
Mouse Eotaxin Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Description
The Mouse Eotaxin(CCL11) ELISA quantitates Ms Eotaxin in mouse serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin. Principle of the method The Mouse Eotaxin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
AHC6054CU recognizes natural and recombinant eotaxin.Specifications
| P48298 | |
| 3.6 pg/mL | |
| ELISA Kit | |
| Mouse | |
| Colorimetric Microplate Reader | |
| Scya11,eotaxin | |
| 5.6% | |
| HRP | |
| RUO | |
| 2°C to 8°C | |
| Mouse | |
| 3 hr. 10 min. |
| 31.3-2,000 pg/mL | |
| Biotin | |
| Serum, Supernatant | |
| ELISA | |
| 20292 | |
| 9.4% | |
| Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 10 x 96 Tests | |
| Serum, 50 μL; Supernatant, 50 μL | |
| Small-inducible cytokine A11, CCL11 | |
| 1 hr. 20 min. |
For Research Use Only