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Mouse CCL3/MIP-1 alpha Quantikine ELISA Kit, R&D Systems


Manufacturer: r & d systems  RDSMMA00

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Catalog No. MMA00

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Description & Specifications

Specifications

Gene ID (Entrez) 20302
Product Type Solid Phase Sandwich ELISA
Packaging Quantity 2 Plates
Quantity 1 Kit
Sensitivity 1.5pg/mL
Sample Type Cell Culture Supernates, Serum
Sample Volume Cell Culture Supernates (50μL), Serum (50μL)
Species Mouse
Specificity Natural and recombinant mouse MIP-1 alpha
Sufficient For Sufficient materials to run ELISAs on at least two 96-well plates

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Assay Type: Solid Phase Sandwich ELISA
Sample Type/Volume Required: Cell Culture Supernates (50μL), Serum (50μL)
Sensitivity: 1.5pg/mL
Specificity: Natural and recombinant mouse MIP-1 alpha
Cross Reactivity: <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.