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Mouse anti-Melan-A/MART-1, Clone: SPM342, Azide Free, Novus Biologicals™


Manufacturer: novus biologicals canada  NBP23476502MG

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 Disclaimers

For Research Use Only

Catalog No. NBP23476502

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Description & Specifications

Specifications

Applications ELISA
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Host Species Mouse
Formulation 10mM PBS. Does not contain BSA. Azide Free.
Regulatory Status RUO
Immunogen Recombinant hMART-1 protein
Primary or Secondary Primary
Format Purified
Concentration 1mg/mL
Conjugate Unlabeled
Gene ID (Entrez) 2315
Quantity 0.2mg
Antigen Melan-A/MART-1
Storage Requirements Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Isotype IgG2b, κ
Gene Accession No. Q16655
Reconstitution Protein A purified
Clone SPM342
Purification Method Purified
Monoclonal or Polyclonal Monoclonal
Test Specificity This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Does not react with Mouse or Rat.
Cross Reactivity Human