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Mouse anti-Melan-A/MART-1, Clone: M2-9E3, Novus Biologicals™

Manufacturer: novus biologicals canada  NBP21519801MG

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For Research Use Only

Catalog No. NBP21519801


Description & Specifications


Antigen Melan-A/MART-1
Applications ELISA
Applications Flow Cytometry
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Clone M2-9E3
Concentration 0.2mg/mL
Conjugate Unlabeled
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Format Purified
Formulation PBS with 0.05% BSA. Protein G purified.
Gene Accession No. Q16655
Host Species Mouse
Immunogen Recombinant human Melan-A protein was used as immunogen to generate the Melan-A antibody.
Isotype IgG2b, κ
Purification Method Purified
Quantity 0.1mg
Regulatory Status RUO
Storage Requirements Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Gene ID (Entrez) 2315
Test Specificity This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.