IL-6 Mouse anti-Human, Clone: 6708, R&D Systems
Mouse Monoclonal Antibody
Manufacturer: R & D Systems MAB206500
Antibody; Primary; Mouse anti-IL-6, Clone: 6708, R and D Systems; 500μg; Unlabeled; Isotype: IgG1; Applications: ELISA Cap, NEUT, WB; Species Reactivity: Hu
Interleukin 6 (IL-6) is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3).
Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).
|Reconstitute at 0.5mg/mL in sterile PBS.|
|ELISA Capture,Neutralization,Western Blot|
|Lyophilized from a 0.2μm filtered solution in PBS with Trehalose. &asterisk;Small pack size (SP) is supplied as a 0.2μm filtered solution in PBS.|
|E. coli-derived recombinant human IL-6|
|2° to 8°C under sterile conditions after reconstitution; 6 months, −20° to −70°C under sterile conditions. Avoid repeated freeze-thaw cycles.|
|Detects human IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL-6, rhOSM, rhLIF, rhIL-11, rhgp130, or rhCNTF.|