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Mouse anti-CD43/Sialophorin, Clone: DF-T1, Novus Biologicals™

Manufacturer: novus biologicals canada  NBP21519001MG

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For Research Use Only

Catalog No. NBP21519001

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Description & Specifications


Host Species Mouse
Antigen CD43/Sialophorin
Storage Requirements Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Applications ELISA
Applications Flow Cytometry
Applications Immunocytochemistry
Applications Immunofluorescence
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Immunogen Myeloblastic KG1 cells were used as the immunogen for this antibody.
Quantity 0.1mg
Format Purified
Clone DF-T1
Formulation PBS with 0.05% BSA. Protein G purified.
Monoclonal or Polyclonal Monoclonal
Gene ID (Entrez) 6693
Cross Reactivity Human
Regulatory Status RUO
Conjugate Unlabeled
Gene Accession No. P16150
Concentration 0.2mg/mL
Isotype IgG1, κ
Purification Method Purified
Test Specificity It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Primary or Secondary Primary