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Molecular Probes™ Rhod-2, AM, Cell Permeant DFS Item

Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells

Manufacturer: life technologies  R1245MP

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Catalog No. R1245MP


Description & Specifications


For Use With (Equipment) Fluorescence Microscope
Quantity 20 x 50μg
Storage Requirements Store in freezer (-5° to -30°C) and protect from light.

Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.

  • Fluorescence signal from these cells is generally measured using fluorescence microscopy
  • Label (Ex/Em of Ca2+-bound form): Rhod-2 (552/581nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 570nM
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling