The Multiplexed Proteomics™ Phosphoprotein Gel Stain Kit contains the Pro-Q™ Diamond phosphoprotein gel stain and the SYPRO™ Ruby protein gel stain. Determining the ratio of the Pro-Q Diamond dye to the SYPRO Ruby dye signal intensities for each band or spot provides a measure of the phosphorylation level normalized to the total amount of protein. Using both stains in combination makes it possible to distinguish a low amount of a highly phosphorlyated protein from a higher amount of a less phosphorylated protein.
Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis
|Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis|