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Invitrogen™ Molecular Probes™ Newport Green™ DCF, Dipotassium Salt, Cell Impermeant DFS Item

Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+

Manufacturer:  Invitrogen™ LSN7990

Catalog No. N7990



Description

Description

  • Kd for Ca2+ >100μM), making this a valuable probe for detecting Zn2+ influx into neurons through voltage- or glutamate-gated channels
  • When used alongside dyes with dual Ca2+/Zn2+ sensitivity such as fura-2 and mag-fura-2, provides confirmation that changes in Zn2+ levels, and not Ca2+ or Mg2+, are being detected
  • The response to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized
  • Label (Ex/Em): Newport Green DCF (approximately 505/535nm)
  • Kd (Zn2+) (in buffer):approximately 1μM
  • Lyophilized product may be dissolved in distilled water or aqueous buffer for use
  • Product is typically loaded into cells using microinjection or diffusion from patch pipet

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Zinc and Heavy Metal Ion Detection

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Fluorescence Microscope
Store at room temperature and protect from light.
Documents

For Research Use Only. Not for use in diagnostic procedures.