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Molecular Probes™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate) DFS Item

Continuously assay acid phosphatases at low pH

Manufacturer: life technologies  D22065

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Catalog No. D22065

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Description & Specifications


Quantity 10 x 10mg
Storage Requirements Store in freezer (-5° to -30°C).
Detection Method Fluorescent
For Use With (Equipment) Microplate Reader
Product Type Substrate, Phosphatase
Excitation Wavelength Range 358/455
Dye Type DiFMU
Color Blue
Target Phosphatases

DiFMUP is a fluorinated 4-methylumbelliferyl phosphate (MUP) derivative that has a lower pKa than that of MUP, making DiFMUP an excellent substrate for continuously assaying acid phosphatases at low pH. The reaction product of DiFMUP has excitation/emission maxima of approx. 358/450nm.

Biochemical Kinase Assays, Biochemical Toxicology Assays, Cell Analysis, Cellular Imaging, Cellular Toxicology Assays, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Kinase Biology, Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation, Target-Based ADME/Tox Assays