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Molecular Probes™ DAF-FM Diacetate (4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate) DFS Item


For detection and quantification of low concentrations of nitric oxide (NO)

Manufacturer: life technologies  D23844

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Catalog No. D23844

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Description & Specifications

Specifications

Quantity 10 x 50μg
Storage Conditions Store in freezer (-5 to -30°C) and protect from light.
Detection Method Fluorescent Dye-Based
Sensor Types Free Radical Sensor

It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

  • Ex/Em of DAF-FM: approx. 495/515nm
  • Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
  • DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for
  • Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution
  • Fluorescence quantum yield of DAF-FM is approx. 0.005, but increases about 160-fold, to ~0.81, after reacting with NO
  • Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
  • Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
  • in vivo imaging of NO in zebrafish
I

  • ntravital microscopic detection of NO generation associated with angiogenesis in mice
  • Quantitation of ATP-induced NO release in rabbit platelets
  • Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5
  • NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture
  • DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx. 3nM versus approx. 5nM for DAF-2)

Refer to the Molecular Probes™ Handbook for additional product information.

Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitric Oxide Research, Nitro-Oxidative Stress