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Invitrogen™ Molecular Probes™ Calcium Green™-5N, AM, Cell Permeant, Special Packaging DFS Item

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+

Manufacturer:  Invitrogen™ LSC3739

Catalog No. C3739



Description

Description

  • Uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues
  • Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
  • Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • These probes are excited by visible light, and because the energy required for excitation is low, potential for cellular photodamage is reduced
  • Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of the spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem

  • Label (Ex/Em): Calcium Green-5N (506/532nm)
  • Fluorescence intensity increase upon binding Ca2+ : approximately 38 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

Fluorescence Microscope
Store in freezer (-5 to -30°C).
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.