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Molecular Probes™ Calcium Green™-1, AM, Cell Permeant DFS Item


Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+

Manufacturer: life technologies  C3011MP

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Catalog No. C3011MP

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Description & Specifications

Specifications

For Use With (Equipment) Fluorescence Microscope
Quantity 500μg
Storage Requirements Store in freezer (-5 to -30°C) and protect from light.

  • They have uses in many calcium signaling investigations, including measuring intracellular Ca2+, following Ca2+ influx and release, and multiphoton excitation imaging of Ca2+ in living tissues
  • Cells may be loaded with the AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
  • Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
  • Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem

  • Label (Ex/Em): Calcium Green; -1 (506/531nm)
  • Fluorescence intensity increase upon binding Ca2+ : approximately 14 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling