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Invitrogen™ Molecular Probes™ ActinRed™ 555 ReadyProbes™ Reagent DFS Item

Selective, high-affinity F-actin probe conjugated to red-orange fluorescent dye tetramethylrhodamine (TRITC)

Manufacturer:  Invitrogen™ LSR37112

Catalog No. R37112



Includes: 2 x 2.5mL dropper bottles

Description

Description

  • Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
  • High-affinity staining of F-actin with superior specificity compared to antibody methods
  • Outstanding fluorescence performance (Ex/Em: 540/565nm)
  • Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
  • Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin
  • Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low nonspecific binding
  • Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability
  • Demonstrating very little nonspecific staining, allows high-contrast discrimination of actin staining
  • Visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations
  • Fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot™ probe
  • Validated and robust reagent for studying cytoskeleton in the context of stem cell differentiation, cancer biology, as marker in RNAi library screens, or target in drug discovery
  • May be added directly to fixed cells in full media or buffer solutions
  • Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until the optimal staining intensity is obtained
  • Detected through standard TRITC (Rhodamine/DiI/Cy3™/Alexa Fluor™ 546) filters

Cell Analysis, Cell Structure, Cytoskeleton

Specifications

Specifications

Confocal Microscope, EVOS™, Floid™ Cell Imaging System, Flow Cytometer, Fluorescence Microscope
Store at ≤ 25°C.
Red-Orange
Documents

For Research Use Only. Not for use in diagnostic procedures.