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Invitrogen™ Molecular Probes™ ActinGreen™ 488 ReadyProbes™ Reagent DFS Item

Selective, high-affinity F-actin probe conjugated to bright, photostable, green-fluorescent dye

Manufacturer:  Invitrogen™ LSR37110

Catalog No. R37110



Includes: 2 x 2.5mL dropper bottles

Description

Description

  • Formulated as a room temperature-stable solution in dropper bottle
  • Tip and drip 2 drops/mL to stain cells for optimally bright and distinct nuclear counter-staining and incubate 15 to 30 minutes in most cases
  • High-affinity staining of F-actin with superior specificity compared to antibody methods
  • Outstanding fluorescence performance (Ex/Em: 495/518nm)
  • No need to dilute, weigh, or pipet
  • Phalloidin is bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin
  • Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding
  • Phalloidin binds F-actin with high selectivity, while Alexa Fluor 488 dye provides green fluorescence of unparalleled brightness and photostability
  • Can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations
  • It is fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot™ probes
  • Role of cytoskeleton in cell division and cell motility makes it central not only to normal, as well as malignant processes (unchecked proliferation and invasiveness); cytoskeletal components are targets of several cancer and anti-inflammatory drugs: validated and robust reagent for related studies
  • May be added directly to fixed cells in full media or buffer solutions
  • Detected through standard GFP and FITC filter

Cell Analysis, Cell Structure, Cytoskeleton

Specifications

Specifications

Confocal Microscope, Floid™ Cell Imaging System, Flow Cytometer, Fluorescence Microscope
Store at ≤ 25°C.
Green
Documents

For Research Use Only. Not for use in diagnostic procedures.