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Thermo Scientific Microphthalmia (Transcription Factor) (MiTF) Ab-1, Mouse Monoclonal Antibody


Ensure accurate, reproducible results in immunohistochemistry experiments with the Thermo Scientific™ Microphthalmia (Transcription Factor) (MiTF) Ab-1, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific  MS771P1ABX

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Catalog No. MS771P1ABX

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Description & Specifications

Specifications

Type Primary
Classification Monoclonal
Research Discipline Transcription Regulation
Concentration 1mg/mL
Antigen Microphthalmia (Transcription Factor) (MiTF) Ab-1
Host Species Mouse
Immunogen N-terminal fragment of human Mi protein
Format Purified
Isotype IgG1/k
Quantity 100µL
Regulatory Status RUO
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Clone C5
Cross Reactivity Canine
Cross Reactivity Equine
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat

Ensure accurate, reproducible results in immunohistochemistry experiments with the Thermo Scientific™ Microphthalmia (Transcription Factor) (MiTF) Ab-1, Mouse Monoclonal Antibody.

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

Host Species: Mouse

Clone: C5

Isotype: IgG1/κ

Species Reactivity: Human, Horse, Dog, Mouse and Rat. Others not known.

Epitope: N-terminal

Immunogen: N-terminal fragment of human Mi protein

Molecular Weight: 52-56kDa doublet

Positive Control: 501 Mel cells, colo32 cells

Cellular Localization: Nuclear

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Gel Supershift

Note:

USA: RUO; Int'l: RUO