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MHC Class II I-Ab Mouse anti-Mouse, eFluor® 450, Clone: AF6-120.1, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS48532080

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Catalog No. 501128789



Description

Description

Description: This AF6-120.1.2 monoclonal antibody reacts with the mouse MHC Class II I-Ab alloantigen of H-2b bearing mouse strains, including C57Bl/6 and 129. This cell surface molecule is involved in antigen presentation to T cells expressing CD3/TCR and CD4. The AF6-120.1 antibody crossreacts with H-2k and H-2u haplotypes. However, reactivity to other haplotypes (i.e., d, f, g7, p, q, r, and s) has not been observed. Applications Reported: This AF6-120.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-120.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC class II.
Specifications

Specifications

MHC Class II I-Ab
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06342
H-2Eb, H2Eb, Ia-4, Ia4, MGC163794, MGC163796
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
AF6-120.1
eFluor® 450
H2-Ab1
Liquid
H2-Ab1
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
14961
Documents

For Research Use Only.