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MHC Class II (I-A/I-E) Rat anti-Mouse, Super Bright 436, Clone: M5/114.15.2, eBioscience™ DFS Item

Rat Monoclonal Antibody

$204.00 - $453.00

Specifications

Antigen MHC Class II (I-A/I-E)
Clone M5/114.15.2
Target Species Mouse
Conjugate Super Bright 436
Isotype IgG2b, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
62532180 View Documents Life Technologies
LS62532180
25 μg Each for $204.00
 
62532182 View Documents Life Technologies
LS62532182
100 μg Each for $453.00
 
Description

Description

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determit present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes. Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye- conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specifications

Specifications

MHC Class II (I-A/I-E)
Mouse
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
M5/114.15.2
Super Bright 436
Flow Cytometry
Primary
Monoclonal
PBS with BSA and 0.09% sodium azide; pH 7.2
RUO