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MHC Class II (I-A/I-E) Rat anti-Mouse, Clone: M5/114.15.2, eBioscience™

Rat Monoclonal Antibody

Manufacturer:  Life Technologies LS14532185

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Catalog No. 501129455



Description

Description

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determit present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes. Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections and in vitro blocking of T cell proliferative responses. (Please use Functional Grade purified M5/114.15.2, cat.16-4321, in functional assays.). Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specifications

Specifications

MHC Class II (I-A/I-E)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04231, P06342
I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, MHC class 2, MHC class II glygoproteins
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Western Blot
M5/114.15.2
Unconjugated
H2-Ab1
Liquid
H2-Ab1, H2-Eb1
IgG2b, kappa
500 μg
4° C
Primary
14961, 14969
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For Research Use Only.