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MHC Class I (H-2Kb) Mouse anti-Mouse, PerCP-eFluor™ 710, Clone: AF6-, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS46595880

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Catalog No. 501129487



Description: This AF6- monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF6- antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6- antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.


MHC Class I (H-2Kb)
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
H-2K, H-2K(d), H2-K, K-f,H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC H2-K transplantation antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I antigen; MHC class I heavy chain H2-K; class I major histocom
Affinity chromatography
Flow Cytometry
PerCP-eFluor™ 710
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!

For Research Use Only.