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MHC Class I (H-2Db) Mouse anti-Mouse, APC, Clone: 28-14-8, eBioscience™

Mouse Monoclonal Antibody

Manufacturer:  Life Technologies LS17599982

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Catalog No. 501128675



Description

Description

Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin. Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
Specifications

Specifications

MHC Class I (H-2Db)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P14426
H2DB, H-2 class I histocompatibility antigen D-B alpha chain, H2D1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
28-14-8
APC
H2-D1
Liquid
H2-D1
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
14964
Documents

For Research Use Only.