Please login to your online account to display your discounted pricing

Thermo Scientific™ Lab Vision™ MGMT (O-methylguanine-DNA methyltransferase) Ab-1, Mouse Monoclonal Antibody


Ensure accurate, reproducible results in immunohistochemistry experiments with the Thermo Scientific™ MGMT (O-methylguanine-DNA methyltransferase) Ab-1, Mouse Monoclonal Antibody.

Manufacturer: thermo scientific™ lab vision™  MS470P1ABX

 View more versions of this product

Catalog No. MS470P1ABX

  • / Each
 

Description & Specifications

Specifications

Host Species Mouse
Applications Immunohistochemistry (Paraffin)
Applications Western Blotting
Immunogen Purified recombinant human MGMT protein
Cross Reactivity Hamster
Cross Reactivity Human
Cross Reactivity Mouse
Cross Reactivity Rat
Isotype IgG1
Antigen MGMT (O-methylguanine-DNA methyltransferase) Ab-1
Quantity 100µL
Format Concentrated
Regulatory Status RUO
Clone MT 3.1
Type Primary
Classification Monoclonal
Conjugate Unlabeled
Formulation Ab without BSA/Azide (Frozen)
Research Discipline Enzyme
Concentration 1mg/mL

Ensure accurate, reproducible results in immunohistochemistry experiments with the Thermo Scientific™ MGMT (O-methylguanine-DNA methyltransferase) Ab-1, Mouse Monoclonal Antibody.

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug-resistant.

Host Species: Mouse

Clone: MT 3.1

Isotype: IgG1

Species Reactivity: Human. Also reacts weakly with Mouse, Rat and Hamster. Others not known.

Epitope: aa 8-221

Immunogen: Purified recombinant human MGMT protein

Molecular Weight: 25kDa

Positive Control: Raji cells, Colon CA

Cellular Localization: Nuclear and cytoplasmic

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Flow Cytometry

Note:

USA: RUO; Int'l: RUO