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For accurate, sensitive, on-column methylation and detection by GC analysis use Thermo Scientific™ MethElute™ Reagent (TMPAH). It is ideally suited for the quantitative methylation and detection of barbiturates, sedatives, xanthine bases, phenolic alkaloids, and phenytoin, giving a single quantitative peak for each substance. When the reagent is heated with drug-containing extracts, serum, or urine the drugs containing reactive amino, hydroxyl, and carboxyl functions will be methylated at the reactive sites.
Accuracy comparable or better than the UV/TLC method (GC is superior to UV/TLC when phenobarbitol and phenytoin present)
High precision, with coefficient of variation of 5% or less
High sensitivity; barbiturates deteced down to 0.2 mg/dL