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MELK Recombinant Human Protein DFS Item

MELK Recombinant Human Protein

Manufacturer:  Life Technologies LSPV4823

Catalog No. PV4823



Description

Description

MELK is a serine⁄threonione kinase which is related to the AMPK family that has been linked to the cell cycle. MELK has also been shown to be overexpressed in cancer and has been identified has a potential target for cancer therapeutics.
Specifications

Specifications

NP_055606.1
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
66.8kDa
95% by SDS-PAGE
–80°C
1 Each
MELK
MELK was pre-diluted in enzyme dilution buffer and assayed in 20mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 1mM Na3VO4, 5mM β-glycerophosphate, 2mM DTT, 0.02% Triton™ X-100, 200μM ATP, 400μg/mL ZIPtide peptide substrate (KKLNRTLSFAEPG) and trace [32P]- γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
CAMKL
MELK Recombinant Human Protein
Human
0.46mg/mL
5610
MELK Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
MELK Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 1-340
2,750nmoles of phosphate transferred to ZIPtide peptide substrate (KKLNRTLSFAEPG) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.083μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.