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Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kits


Synthesize long cDNAs up to 20 kb at elevated temperatures (up to 65°C) with this complete system for highly efficient synthesis of first strand cDNA.

Manufacturer: thermo scientific™  K1652

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 Disclaimers

This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.

Catalog No. FERK1652

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Description & Specifications

Specifications

For Use With (Application) First Strand cDNA synthesis; RT- PCR; RT-qPCR; Construction of cDNA libraries; Generation of probes for hybridization; RNA amplification.
Product Type RevertAid™ Premium First Strand cDNA Synthesis Kit
Sufficient For 100 reactions

Maxima H Minus First Strand cDNA Synthesis Kit† is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

The new Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Highlights

  • Integrated genomic DNA removal step
  • Increased reaction temperatures -the first strand of cDNA can be synthesized within the 42 to 65°C temperature range
  • High yields of full-length first strand cDNA -with RNA templates up to 20 kb
  • Flexible priming -oligo(dT)18, random hexamer, or gene-specific primers