Marginal Zone B Cells Monoclonal Antibody (HIS57), eBioscience™, Invitrogen™

Description

Description: The monoclonal antibody HIS57 recognizes an unknown antigen expressed by rat marginal zone B cells. Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low. Applications Reported: This HIS57 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: This HIS57 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Marginal zone B cells are an anatomical B cell compartment located in the spleen at the area between red and white pulp. Marginal zone B cells serve as a first line of defense against blood-borne antigens in a T cell-independent manner. Marginal zone B cells can also be phenotyped as B cells that are IgM high, IgD low, CD21 high and CD23 low.