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MAP3K2 (MEKK2) Recombinant Human Protein DFS Item

MAP3K2 (MEKK2) Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3822

Catalog No. PV3822



Description

Description

MAP3K2 (MEKK2) is a serine⁄threonine kinase that activates kinases involved in the MAP kinase signaling pathway.
Specifications

Specifications

AAF63496.3
Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target and lead identification and validation
97.2kDa
90% by SDS-PAGE
–80°C
1 Each
MAP3K2 (MEKK2)
MAP3K2 was assayed in a two stage coupled reaction involving cascade phosphorylations of inactive MAP2K1 Wild-Type (P3093) and inactive MAPK3 (PV3312) followed by phosphorylation of myelin basic protein (MBP) substrate by activated MAPK3. The enzymes were diluted in enzyme dilution buffer before adding to the reaction. In stage one, MAP3K2 (8.33μg/mL final concentration) is incubated with inactive MAP2K1 (33μg/mL final concentration) and inactive MAPK3 (33 μg/ml final concentration) in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100 and 100 μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix is transferred to 25μL of a reaction mix containing 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100 μM ATP, 500μg/mL MBP substrate and trace [ 32P]-γ-ATP and incubated for 10 minutes at 30°C
Kinases & Inhibitors
STE11 kinase family
220nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein Activity determined at a final protein concentration of 1.39μg/mL
GST Tag
0.41mg/mL
10746, 6773
MAP3K2 (MEKK2) Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
MAP3K2 (MEKK2) Recombinant Human Protein
Kinase assay
Baculovirus
MAP3K2 (MEKK2) Recombinant Human Protein
Human
Documents

For Research Use Only. Not for use in diagnostic procedures.